Nitrogen-cycle genes and transcripts abundances under agricultural management practices in a long-term continuous cotton field - NIFA_Soil_N-cycle_qPCR_Data_hjl.csv

https://doi.org/10.7290/7WP5rsToDb

Hu, J. DeBruyn J. (2021). Nitrogen-cycle genes and transcripts abundances under agricultural management practices in a long-term continuous cotton field. TRACE Data Sets. https://doi.org/10.7290/7WP5rsToDb.

Including three datasets: 1) primary data; 2) relative abundance data; 3) absolute abundance data; and 4) transcript-to-gene ratio data. 1) The primary qPCR dataset includes five N-cycle genes and gene transcripts, as well as 16S rRNA gene and 16S rRNA. These data were normalized to extracted DNA (for N-cycle genes) or RNA (for N-cycle gene transcripts); 2) The relative abundance dataset includes five N-cycle genes and gene transcripts normalized to 16S rRNA gene and 16S rRNA; 3) The absolute abundance dataset includes five N-cycle genes and gene transcripts, as well as 16S rRNA gene and 16S rRNA. These data were normalized to soil weight (per gram dry weight soil); 4) The transcript-to-gene ratio dataset includes the value of transcript-to-ratio of five N-cycle genes based on absolute abundances of N-cycle genes and transcripts. The transformation methods of datasets in this file were described in section METHODOLOGICAL INFORMATION #2.

Soil nitrogen
N cycle
Soil microbes
Quantitative PCR (qPCR)
Quantitative reverse transcriptase PCR (qRT-PCR)

DeBruyn, Jennifer (Depositor)
DeBruyn, Jennifer (Creator)
Hu, Jialin (Creator)

2018-06